Sentence examples for restricted upstream from inspiring English sources

The downstream region was ligated into pGEM-T Easy (Promega) followed by the SpeI/XhoI restricted upstream region and the SalI restricted orotidine-5′-monophosphate decarboxylase-encoding gene pyr4 as a selection marker, resulting in pBM1.

Our study was restricted to the 5' upstream regions of each gene in consideration, and did not consider 3' regions where cis-regulatory signals can also, presumably, exist.

However, due to incompleteness of the sequenced Atlantic cod genome and fragmentary nature of its assembly, our analysis was restricted to 11 upstream regions of pre-miRNAs.

Again, RNA polymerase II was mostly restricted to the upstream part of the gene in wild-type cells grown under repressing conditions, but extended to the entire URA8 open reading frame in rpb1-L1397S.

For example, the expression domain of SHOOT MERISTEMLESS (STM), an H3K27me3-target gene in all five data sets, is restricted by an upstream sequence that is conserved between monocots and dicots (Uchida et al. 2007).

Our findings suggest that the action of kinases at origins is restricted not only upstream of their action (for example, at recruitment) but also after phosphorylation of their target(s) has occurred.

This led to very low overall plant densities at 16 trees ha−1, restricted to wetter zones upstream of retention dams.

According to the above promoter analysis, the promoter region of DNA-PKcs was subsequently restricted to position −220 upstream from the DNA-PKcs initiation site, cloned into the luciferase reporter plasmid and examined for promoter activity 72 h after E2 stimulation, confirming the relevance of the hypothesized EREs in DNA-PKcs transactivation.

GFP expression driven by the PB103464.00.0 5' upstream region was restricted to the liver stage (Figure 2B, upper panel), confirming that the chosen 5' upstream region indeed contains a liver stage-specific promoter.

However, increasing agriculture activities, shrimp farming and human settlement, uncontrolled deforestation, restricted fresh water supply from upstream due to the Farakka barrage, increasing river siltation, unscientific changes in land use, and climate change all still pose threats to the water quality of the river as well as to the whole mangrove ecosystem.

The region corresponding to the binding site sequence search was restricted to a 2 kb upstream region from the TSS.