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In contrast to terminally differentiated cell types, which mount restricted responses to various stimuli, ES cells mount essentially infinite responses, differentiating to all cell types in the organism.
Our previous [10] and present results showing HLA-I binding 9mer peptides capable of activating CD4+ T cell dependent responses, may suggest that some of the "alternative restricted" responses described by Frahm et al. [22] reflect CD4+ T cell recognition of epitopes restricted by HLA-II.
In other individuals, peptide 16 induced the dominant DRB1*1501 restricted responses (Figure 2E).
As indicated from data in Table 3 and 4 only half of the HLA-II restricted responses were observed in more than one donor whereas four of the five HLA-I restricted responses were observed in at least two donors.
Attempts to block HLA-I and HLA-II restricted responses were performed in 4 cultures of 10 days expanded cells.
However, only 5 peptides induced strictly CD8+ T cell dependent HLA-I restricted responses whereas 16 peptides induced CD4+ T cell dependent responses.
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Restricted response categories were included, where possible, to allow for more straightforward analysis.
Table 3 Summary of responses to the questionnaire (restricted response categories only) Question Responses A lot Moderately A little Not at all Unsure In general, how well did your initial reporting decisions correlate with the CADx output?
In some individuals, peptide 19 induced the DRB1*1501 restricted response (example in Figure 2B).
In these practice sessions the restricted response times used (1365 ms) were found to be of sufficient duration for the observers to undertake the tasks comfortably].
This restricted response to VPA is surprising in view of the global histone hyperacetylation detected in preimplantation embryos and ES cells exposed to HDAC inhibitors [11].
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