The application of this concept is very much based on literature published in high income countries, with restricted relevance and adequacy in a Sub-Saharan Africa countries with limited resources and health coverage like in Cameroon [ 37].
To return to the discussed difficulty of p53 signaling studies in leukemia cell lines, the presented technique will enable routine investigation of primary childhood ALL cells that have been performed so far mainly in childhood ALL cell lines with restricted relevance to the clinical setting due to inherited mutations i.e. in CEM and JURKAT leukemia cells [ 13, 16].
In particular, it is often not possible to extrapolate the results to a reference population, thus greatly restricting their relevance.
Therefore, the use of T. reesei strain SVG17 was fully in line with the concept of the study, and the limitation in beta-glucosidase noted was not considered to restrict the relevance of the current investigation.
ELISA screening (for lower avidity anti-dsDNA antibodies) followed by risk stratification through the use of more stringent assays (Farr, EliA, Crithidia) and restricting the relevance of anti-dsDNA antibody presence to specific clinical manifestations might provide us with a better index of the pathogenicity of anti-dsDNA antibodies.
If so, should such knowledge expand in parallel with other biomedical information, or should any such expansion be restricted by its relevance to changes in clinical performance (the 'barefoot doctor' model)?
Having shown that maximal release of NETs is achieved within 24 hours after CLP, this confinement of bacteria by NETs is obviously temporally restricted and their relevance in the late phase of sepsis seems to decline.
The second limitation was that the environmental study from which the data for this study were derived focused on airway diseases and atopic manifestations and, therefore, the selection of living conditions of the 6-year old children was restricted to their relevance for these health outcomes.
From an in-silico meta-analysis of available primary data of gene expression profiles on human MTCL we cross-reference genes reported to transform T-cells in mice and reflect on their general vs entity-restricted relevance and on target-promoter influences.
The uncertain future of sharing sits in between these two forces: vital startups that brave ahead in spite of regulation uncertainties and multinational behemoths who need to preserve their economic relevance while restricted by their current business models.
Although the insights obtained from this data set are highly informative in helping to determine key regions and their likely drivers, the interpretation of biological relevance is restricted to individual gene information, which is often of limited value since it is possible to construe a cancer-related function for most genes.
