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For this purpose, a regional approach is most efficient, as it enables the use of a restricted reference database of trusted sequences for all species of a specific region or ecological community [21], [46].
We mapped the RNA-seq data from two biological replicates of the JKM139 wild-type strain grown in raffinose (time zero, T0) and shifted to galactose-containing medium for 60 (T60) and 240 (T240) min on a restricted reference 'genome' corresponding to the MAT locus ±10 kb.
Note that the latter procedures except 17 are regularly performed in canton Vaud (cumulated proportion of 6.9%, see table 3); cystoscopic surgery for urethra structure or bladder tumor (table 3, 17) has been considered suitable for day care for a long time, although it was more frequently performed as inpatient care in canton Vaud and not included in the restricted reference list [ 31].
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The field of epigenetic being extremely prolific, we have restricted our reference list to the essentials.
However, most in-house methods are restricted to reference laboratories, and hence are not commonly employed around the globe.
Real-time RT-PCR is the most sensitive and specific method, but remains restricted to reference laboratories.
We restricted the reference database to patients aged >15 years, with complete data and 30-day follow-up.
A typical SNP and INDEL calling pipeline based on alignment to a reference is restricted by the reference.
We have therefore restricted our references to a possible role in geometry to speculation.
Searches were restricted to references published since 1980 involving only human subjects.
During this process, we restricted probe-reference mismatches to a maximum of 25%.
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