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The following limitations have restricted its applications: (i) It is expensive.
That has restricted its applications to things for which there is no alternative.
Nevertheless, the poor solubility of chitosan in neutral or alkalinized media has restricted its applications in the pharmaceutical and biomedical fields.
Due to the low stability of isoC under alkaline conditions, the 5-methyl derivative (isoCMe) was used instead.[ 9] This unnatural isoG:isoCMe base pair was studied by S. Benner,[ 10] and evaluated in PCR amplification showing 96%% fidelity.[ 11] Results from these studies revealed that the pairing selectivity in DNA was not adequate and restricted its applications.
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However, its poor adhesion to most smooth or non-porous substrates has restricted its application.
As a result, standard substance was indispensable for identifying the targets, which restricted its application in qualitative analysis.
However, chemical structure information of analytes can't be obtained by the methods, which greatly restricted its application for qualitative analysis.
However, data analysis of high-throughput sequencing techniques requires highly trained specialists, which has restricted its application in large-scale biomonitoring efforts (Seong Woon et al. 2010).
However, inferior cycling performance and rate capability owing to the poor ionic and electronic conductivities have greatly restricted its application in ALIBs.
However, Mg's high rate of degradation leads to an excessive inflammatory response and this has restricted its application in bone tissue engineering.
This produced a urine blank that restricted its application to the levels found during the ovulatory cycle.
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CEO of Professional Science Editing for Scientists @ prosciediting.com