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Data acquired from the restricted fed mice, ad libitum fed mice, and the fasted restricted fed mice were normalized to acidic ribosomal phosphoprotein PO (36B4).
However, Nocturnin is acutely induced by the absence of the expected meal in eWAT of restricted fed mice.
The observation that Nocturnin is induced by fasting in restricted fed mice is the first example of Nocturnin's acute induction in the mouse.
Interestingly, Nocturnin becomes rhythmically expressed in the white adipose tissue of restricted fed mice, indicating that its rhythmic expression pattern is driven by the temporal availability of food.
However, in the white adipose tissue of restricted fed mice, Nocturnin expression was induced approximately two-fold at ZT7 in response to the fast (Figure 4B, right panel).
The finding that Nocturnin is induced by a fast in white adipose tissue of restricted fed mice is contrary to the original hypothesis that Nocturnin would be induced by feeding.
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Cows were restricted-fed during measurements and mean DM intake was 15.0 ± 0.16 kg/d.
The tissue specific expression patterns of core clock genes in restricted-fed animals raises the possibility that the regulation of their expression may be achieved through unknown mechanisms or may be the result of different entraining signals.
Calves fed milk ad libitum by artificial teat had fewer days with diarrhea compared to restricted, bucked-fed calves [ 7].
Restricted-fed cows had lower concentrations of glucose and insulin and increased concentrations of NEFA in plasma during the dry period.
Also, we show that Nocturnin is acutely induced by fasting in the white adipose tissue of restricted-fed mice.
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