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If human-specific testis restricted expression is confirmed, RPS4Y2 would compile all the characteristics mainly associated with human speciation – testis-specific expression and human-specific expression pattern and function [ 29].
Our findings described here demonstrate that restricted expression is also obtained in mature human beta cells.
Indeed, such restricted expression is a useful criterion that has already been helpful in identifying specific olfactory genes such as olfactory receptors and Odorant-Binding Proteins (OBPs) in various species [31].
The endogenous Hprt locus is widely expressed [ 40, 41], nevertheless, it is considered a relatively neutral docking site since the expected restricted expression is observed from tissue-specific promoters inserted at the locus [ 5, 55- 60].
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Restricted expression was not yet completed by P12, since a few cells were still expressing Msx1 along the length of arteries (Fig. 2A, arrowheads), but was achieved at P14 (Fig. 1D,D′).
Unlike other caspase family members, caspase-14 shows restricted expression, being found only in epidermis, skin appendages and thymic Hassall's bodies.
Tissue-restricted expression is a desirable property for candidate genes as therapeutic targets in cancer, since their function could be inhibited without damaging normal tissues, their promoters could be used to enhance expression of cell death-inducing proteins in tumor cells, and tumor-specific proteins could serve as targets for immunotherapy or site-specific delivery of antitumor agents.
The basis for this liver-restricted expression is not completely understood.
It is worth noting that tissue-restricted expression is not the sole contributor of tissue-specific PA usage.
Since the liver-restricted expression is a common feature of most, if not all, mammalian HL genes, we hypothesize that the regulatory elements responsible for liver-specific expression are conserved among mammals.
At CS15 (33 dpc) restricted FRMD7 expression is seen within the outer neuroblastic layer (Fig. 1).
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