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The reported reasons for previous ML failure were as follows: restricted mouth aperture of below 25 mm, major abnormality of anterior neck structure, severe cervical spine ankylosis, previous causes combined with post-surgery/radiation sequels or unknown non-tumoral causes.
However, the faster decrease in signal intensity for a FOV >150 μm could be the result of a restricted clear aperture of the micro-lenses.
Pigment rings restrict the aperture in spider eyes.
But since the pigment rings restrict the aperture there are no reasons to believe that spatial resolution is much impaired after molting.
In crabs, the endopodites of the third pair of maxillipeds meet at the midline and by rotation at their bases can widen or restrict the aperture of the chamber around the mouth.
The amount of light entering the eye is restricted by the aperture in the iris, the pupil.
Narrow field angle scanning is restricted to the numerical aperture or cone angle of the laser beam probing the specimen which increases the error in determining the three-dimensional properties of a specimen.
Especially, the mid-to-peripheral visual field may be affected by a KAMRA, as it has been shown for other aperture restricting optical implants such as keratoprostheses [ 36].
For the example shown in Fig. 7 we can observe that the effective angular aperture of the telescope is restricted to a cone of ±20° around r0,0.
Since the excitation volume is restricted to a diffraction-limited point, no confocal aperture is needed – the generation of out-of-focus background is quite limited – and all the excited photons can be collected and measured.
The opaque Al film restricted the light to be primarily within and above the aperture, causing the transmitted illumination profile to be determined by the aperture properties rather than by the diffraction limit.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com