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The use of HupA revealed that AChE normally restricts trophic ACh actions.
The CMV-AChE-SV40 cassette in pCA13-AChE was restricted by BglII and inserted into pZD55 shuttle plasmid to generate pZD55-AChE.
When a narrowed coronary artery restricts blood flow to the heart muscle, people often feel a dull, aching pressure across the chest or in the neck that lasts for minutes.
The PCR fragments were restricted by HindIII and XbaI and inserted into pCA13 shuttle plasmid to form pCA13-AChE.
My bones and/or joints have ached 7. I've found it difficult to carry out my daily activities 8. I've restricted some of my leisure activities 9. I've restricted what household chores I do 10.
The aching.
Legs start aching.
Just an aching arm.
Oh, my aching ribs.
The enormous aching".
"My heart is aching".
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