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A small number of sequences (12) were shorter than 750 bp and were removed prior to phylogenetic and clustering analysis in order to restrict the dataset to accessions comprising (almost) full-length ITS1 and ITS2 segments.
Our strategy to perform the experiment during three seasons allowed us to restrict the dataset of modulated genes to those more likely to be ripening-specific.
For the homology search, considerably low BLAST scores (≤ 1x10-160) were chosen to restrict the dataset to likely ortholog and paralog candidates.
After applying the specified case definitions to restrict the dataset to individual opioid users, we used descriptive statistics to examine the demographic and geographic characteristics of cases identified in the CDS (drug services) and SPARCS (hospital-based medical services) data.
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To test this assertion we restricted the dataset to very large magnitude eruptions (M≥5.0), and redid the calculation.
After restricting the dataset to one point from every 16 km2 panel, mean wind speed was found to be correlated with elevation (0.48, 0.86), but less well with topographic exposure indices (0.17 0.72).
As observed previously, subtler differences can be detected by restricting the dataset to the family level, if enough genomes are available for a particular family.
Restricting the dataset in this way allowed us to exclude breeding events where we would not be able to identify cases of very close inbreeding (f≥0.125) [13], [14], [30].
We therefore performed the analyses of AOC in relation to age by restricting the dataset to all and only the individuals that were recorded to have survived up to age 2 (i.e. excluding those that were recorded to have survived to age 1 or ≥3) (n = 116 observations).
Hence, we restricted the dataset to Caucasian females which represented the largest proportion of the dataset.
By restricting the dataset to side views the angle assignment is more reliable, and these views are sufficient to generate the full 3D structure.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com