Sentence examples for restraints were generated in from inspiring English sources

Secondary structure restraints were generated in ProSMART (Nicholls et al., 2012 ), and nucleic acid base-pairing and stacking restraints were generated as before (Amunts et al., 2014 ) and were maintained throughout refinement to prevent overfitting.

Secondary structure restraints were generated in ProSMART (Nicholls et al., 2012), and nucleic acid base-pairing and stacking restraints were generated as before (Amunts et al., 2014) and were maintained throughout refinement to prevent over-fitting.

Distance restraints were generated from cross-peaks in 75 ms mixing time NOESY spectra using (1/ r) scaling.

In addition, backbone phi and psi dihedral angle restraints were generated from Cα and Cβ chemical shifts using the program TALOS+ [ 37].

Distance restraints were generated for atom pairs defined as (i, j) where atom i is in TM11 (residues 536 558) or TM12 (residues 572 599) and atom j is in TM3 (residues 159 192) or TM10 (residues 488 516).

Dihedral angle restraints were generated from backbone chemical-shift data using TALOS [ 20].

The structure of lactose and the accompanying refinement restraints were generated using the CCP4 program Monomer Library Sketcher.

Distance restraints were generated from spectra with mixing times between 50 and 150 ms, to minimize contributions from spin diffusion.

Secondary structure restraints for the Sec61 channel were generated in ProSMART (Nicholls et al., 2012).

methylotrophus W3A1 MDH structure, and the library of restraints was generated using the eLBOW program available in Phenix.

Restraint files were generated based on the interaction interface.