Dihedral angle restraints were generated from backbone chemical-shift data using TALOS [ 20].
The structure of lactose and the accompanying refinement restraints were generated using the CCP4 program Monomer Library Sketcher.
Secondary structure restraints were generated in ProSMART (Nicholls et al., 2012 ), and nucleic acid base-pairing and stacking restraints were generated as before (Amunts et al., 2014 ) and were maintained throughout refinement to prevent overfitting.
Secondary structure restraints were generated in ProSMART (Nicholls et al., 2012), and nucleic acid base-pairing and stacking restraints were generated as before (Amunts et al., 2014) and were maintained throughout refinement to prevent over-fitting.
Distance restraints were generated from spectra with mixing times between 50 and 150 ms, to minimize contributions from spin diffusion.
Distance restraints were generated from cross-peaks in 75 ms mixing time NOESY spectra using (1/ r) scaling.
Hydrogen bond restraints (two per hydrogen bond) were generated by a combination of H/D exchange data, medium-range NOEs, and chemical shift index.
methylotrophus W3A1 MDH structure, and the library of restraints was generated using the eLBOW program available in Phenix.
Most opportunities, though, were generated by individuals.
Mononucleosomes were generated by salt dialysis [81].
