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Structural restraints were extracted from 13C- and 15N-edited three-dimensional NOESY spectra.
Distance restraints were extracted from two-dimensional CHHC, PAR, PAIN, DARR, and PDSD spectra[ 24– 27].
A total of 48 restraints were extracted from the spectra and classified as 22 intermolecular and 26 intramolecular restraints (Table S1 and Figure S6).
Distance restraints were extracted from 2D NOESY, 2D H N CPMG-NOESY, 3D N-edited NOESY-HSQC, and 3D N/C-edited NOESY-HSQC spectra recorded in H2O and 3D C-edited HMQC-NOESY spectra collected in D2O.
Similar(56)
Using an iterative assignment and structure calculation protocol, a large number of distance restraints was extracted from 1H/1H mixing experiments recorded on a single uniformly labeled sample under magic angle spinning conditions.
We report a "top down" method that uses mainly duplexes' global orientations and overall molecular dimension and shape restraints, which were extracted from experimental NMR and small-angle X-ray scattering data, respectively, to determine global architectures of RNA molecules consisting of mostly A-form-like duplexes.
Data on physical restraints and residents' characteristics were extracted from residents' records (11/2013 to 2/2014).
Structures were extracted every 40 ps giving rise to an ensemble consisting of 1475 conformations which we subsequently used to generate synthetic NOE restraints.
A much larger number of distance restraints could be extracted from a decoupled NOESY spectrum, because signal overlap is greatly reduced.
The bullet was extracted.
Axial and rotational restraints were applied by a restraint beam.
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