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A letter, sent to the Youth Justice Board (YJB), G4S, Social Services Inspectorate and a Home Office minister by a youth justice academic on behalf of the whistleblowers, alleges children had been badly hurt when guards tried to restrain them, including one boy having his shoulder dislocated, and that staff were encouraged to falsify restraint statistics.
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Detailed restraints and structural statistics of C-CD147EC are summarized in Table S1.
Xlink Analyzer enables automatic visualization of cross-links, identifies cross-links violating spatial restraints, calculates violation statistics, maps chemically modified surfaces, and allows interactive manipulations that facilitate analysis of XL-MS data and aid designing new experiments.
A summary of the restraints and structural statistics for this ensemble is provided (Table S1).
A summary of the restraints and structural statistics for this ensemble is provided in Table S1 and the coordinated structures have been deposited in the Research Collaboratory for Structural Bioinformatics Protein Databank (http://www.rcsb.org/pdb/) under accession code 2JUY.
The NMR restraints and structural statistics are summarized in Table 1.
Tables of chemical shift assignments, distance and dihedral angle restraints, structure calculation statistics and H−P HETCOR, natural abundance H−N HSQC, TOCSY, SNOESY, and NOESY spectra, and results of an unrestrained molecular dynamics simulation of 5′G AGC/3′C GAG.
The use of external restraints (as well as NCS restraints) was vital to achieving reasonable statistics and ProSMART (Nicholls et al., 2012) was found to be the most effective choice in our case.
A summary of structural restraints used in the structure calculation and statistics for the structure ensemble is listed in Table S1.
Table 1 summarizes the restraints that were used and the refinement statistics.
The use of noncrystallographic symmetry restraints between protomers and the optimization of target weights for geometry and B factor restraints greatly improved model quality and refinement statistics.
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