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With the advent of optogenetics, the head-restrained procedure allows for these recordings to be coupled with cell-type specific silencing and activation, free from electrophysiological artifacts.
We describe an intensity-restrained optimization procedure for refining approximate structures of ligands within the protein binding pockets using STD-NMR intensity data on reversibly forming weak complexes.
The energy was minimized using a steepest gradient method to remove any close contacts before the system was subjected to a two-phase position-restrained MD equilibration procedure.
During the ordeal, she was subjected to force-feeding, injections, hair shaving and other uncomfortable procedures -- often while restrained -- reported the International Business Times.
In this study, a direct displacement design procedure for steel frames with buckling-restrained braces (BRBs) is presented.
An energy-based seismic design procedure for framed structures with buckling-restrained braces is proposed using hysteretic energy spectra and accumulated ductility spectra.
This independent validation of the design is based on simulated annealing and restrained molecular dynamics simulation in vacuo, comparable to procedures used to refine NMR and X-ray structures.
Energy dissipation capacity and earthquake response of steel structures installed with buckling-restrained braces (BRB) were investigated, and a straightforward design procedure to meet a given target displacement was developed.
Cephalic vein blood samples were collected from unanesthetized, cage- restrained animals that were trained to present an arm for the procedure.
Analytical models and a performance-based seismic design procedure were developed for a structure with buckling-restrained knee-braces.
In others, restrained simulations are used throughout all the process.
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