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Reimbursements could be raised, restoring viability to the trust fund and bringing better services to all.
Co-treatment with FOH (mixed isomers) protected against ZOL-induced apoptosis, restoring viability to approximately 90% of control cultures.
As in the other assays, eGFP-VasR644A was fully functional with respect to restoring viability to vas embryos, while eGFP-VasΔ655-661 rescued viability at a substantially lower rate than wild-type (p = 0.00054).
We found that Scc2 variants mutated at conserved residues contacting either the concave or the external surface of Scc4 were less effective than wild-type Scc2 in restoring viability to an Scc2-degron (SCC2-AID) strain under depletion conditions.
Lastly, the substitution of glutamine with the albeit smaller (105.1 Da) serine that is biochemically similar as a polar neutral a.a. provided very little improvement compared to the Sup− in restoring viability to λ Dam15 and generated comparatively very small phage, and pinpoint plaques in the absence of gpD or gpD::eGFP complementation.
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Likewise, cell viability was inhibited by H2O2 treatment, and DHF treatment restored viability, but the restoration of cell viability by DHF was blocked by treatment with PI3K or Akt inhibitor).
SupD phage preparations carrying the gpDQ68S allele were only a third that of wild-type and SupF preparations that likely possessed a full complement (405 420 gpD) as compared to SupD phage derivatives that were likely unstable and minimally decorated to restore viability to some progeny, also explaining the poor restoration of phage viability attributed to the gpD68S derivative.
Surprisingly, the addition of an amino or methyl substituent to the 5-position of 4c (compounds 6a and 6b, respectively) appeared to restore viability.
We asked whether overexpression of TUB4 mutants will restore viability.
If present, such donors would be expected to restore viability to a dprE1 mutant.
Indeed, overexpression of dominant negative CHOP construct and extinction of CHOP partially restored viability in Scd1-depleted cancer cells.
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