Sentence examples for restoring translation from inspiring English sources

10.7554/eLife.05033.010 Figure 3. ISRIB addition rapidly dissolves pre-formed stress granules in live cells restoring translation.

Moreover, live cell imaging revealed that ISRIB addition can trigger a remarkably fast dissolution of phospho-eIF2α-dependent SGs in stressed cells, restoring translation.

Given the known neuroprotective effects of restoring protein synthesis rates in prion-diseased mice downstream of misfolded PrP accumulation [ 11, 23], we first asked if restoring translation through PERK inhibition in tau transgene-expressing mice was similarly neuroprotective.

Within a few minutes after administration, ISRIB reverses the effects triggered by eIF2α phosphorylation dissolving RNA stress granules and restoring translation of inhibited mRNAs while reversing de-repression of uORF-containing mRNAs (Sidrauski et al., 2015).

Restoring translation through normalizing eIF4E phosphorylation primarily affected Mmps (Mmp9, Mmp2, Mmp3, Mmp7, and Mmp24) and Dlg4 (PSD-95), but not other FMRP targets such as Camk2a (CamKIIa), Shanks, or Map1B.

Notably, expression of E3 protein partially restored translation of ΔDLP virus mRNA that was strongly inhibited due to eIF2 phosphorylation in non induced cells.

Addition of wild-type eIF4AI does not rescue the inhibition by hippuristanol, whereas eIF4AIIG/T restored translation to ∼60% of normal levels (Fig. 4B, compare lanes 6 and 4 to 5 and 3, respectively).

Once the stress is resolved, PPP1R15A/GADD34 is induced to restore translation by dephosphorylating eIF2α (3).

GADD34 induction leads to a reduction of eIF2α-P, allowing cells to restore translation (Novoa et al., 2001).

This is in contrast to GSK2606414, which restores translation rates to ~100% in vivo and 90% in vitro.

The MEK inhibitor U0126 prevented the decrease in TFAM protein levels in MPP+-treated cells, and restored translation of the mtDNA-encoded proteins.