The over-expression of COQ8, which encodes a putative regulatory kinase, has been shown to restore steady state levels of several Coq polypeptides [10] and the assembly of the high molecular mass CoQ-synthome [8].
Expression of wild-type mtPAP (+LVMTPAP) in the homozygote mutant cell lines was sufficient to restore steady-state levels of mtDNA gene products at both the mRNA and protein level (Supplementary Material, Fig. S2A and B).
Rapid up-regulation to previous expression level was observed after restoring previous steady state conditions.
By quantifying these effects, the partial internal response coefficients allow the assessment of their importance in restoring the steady state [ 32, 62], i.e. (13) According to the connectivity theorem the sum of the partial internal response coefficients for each intermediate is -1.
Second, the deterministic simulations suggest that the three step loop is not as successful at restoring the steady state output as was suggested by the stochastic simulations (deterministic predicts C = 0.92 versus C = 0.96 for stochastic from time 0 to 1000 ms).
Although the case is on appeal, everyone expects that Waterbury will be forced to restore a steady flow year-round.
In this context, partial internal response coefficients allow for the assessment of the relevance of individual reactions in counteracting a perturbation in order to restore the steady state.
As described above, we previously showed that overexpression of VARS2 could partially restore the steady state levels of mt-tRNAval resulting from the m.1624C>T mutation (Rorbach et al, 2008).
Recent work has shown that the over-expression of the putative Coq8 kinase in certain coq null mutants allows synthesis of late-stage Q-intermediates, restores steady-state levels of Coq polypeptides, and their association in a high molecular mass complex known as the CoQ-synthome [8,10,28].
Under frequency load shedding is an effective approach to maintain or restore the steady-state operation of the power system when frequency accidents occur.
