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SupD phage preparations carrying the gpDQ68S allele were only a third that of wild-type and SupF preparations that likely possessed a full complement (405 420 gpD) as compared to SupD phage derivatives that were likely unstable and minimally decorated to restore viability to some progeny, also explaining the poor restoration of phage viability attributed to the gpD68S derivative.
Surprisingly, the addition of an amino or methyl substituent to the 5-position of 4c (compounds 6a and 6b, respectively) appeared to restore viability.
We asked whether overexpression of TUB4 mutants will restore viability.
If present, such donors would be expected to restore viability to a dprE1 mutant.
In E. coli, mutations that restore viability to a thermosensitive DNA elongation mutant (dnaX2016) have previously been isolated [64], [65].
In analogy to bacteria, only a quadruple yeast mutant is non-viable, and a single glutaredoxin or thioredoxin is able to restore viability [31].
Similar(27)
Likewise, cell viability was inhibited by H2O2 treatment, and DHF treatment restored viability, but the restoration of cell viability by DHF was blocked by treatment with PI3K or Akt inhibitor).
Reimbursements could be raised, restoring viability to the trust fund and bringing better services to all.
Huntingtin neurotoxicity suppressors identified through our screen also restored viability in an in vivo Drosophila Huntington's Disease model, making them attractive candidates for further therapeutic evaluation.
Conversely, introduction of a plasmid borne copy of flipR restored viability of the bacteria compared to either the ΔflipR mutant or the ΔflipR mutant harboring the empty control plasmid (Fig. 3i).
Indeed, overexpression of dominant negative CHOP construct and extinction of CHOP partially restored viability in Scd1-depleted cancer cells.
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