Exact(2)
However, while this did reduce plasmid copy number as assessed by yield from plasmid preparation procedures, it did not restore transformation into BL21 under standard conditions (Additional file 2: Figure S2A and S2B).
We hypothesised that if wild-type E1A targeted APC/C function directly during transformation through binding CBP/p300, then it might be possible to restore transformation potential to a transformation-defective E1A species, such as R2G E1A, by interfering with normal APC/C function through the selective knockdown of APC5 and/or APC7 protein levels by RNAi.
Similar(57)
Experiments show the architecture proposed can restore planar transformations with wonderful robustness and effectiveness.
Figure 4B shows that the ΔhrpB2 strain was unable to elicit disease symptoms and that the virulence of the mutant strain was fully restored by transformation with pUFR047_hrpB2.
Flagellar motility can be restored by transformation of the mutant with the wild-type RSP3 gene [ 6], thus providing a nice biological assay for activity of the promoter driving RSP3 transcription.
Figure 2 clearly shows that the wild-type phenotype (low invertase activity under high-glucose conditions) was restored after transformation of the double-mutant Δ hxk1Δ hxk2ADH1p::GLK1 strain with the wild-type (HXK2) or the mutant (HXK2 wca ) genes.
However, the helicase dead mutant K236R DDX21 failed to restore the transforming activity of RasV12, implying that DDX21 helicase activity was required for effective RasV12 transformation.
America's aim is not just physical reconstruction but a social transformation to restore the elders' authority.Another human factor is at play, too.
This transformation will restore natural ecological relationships in the forest ecosystems in south Poland and other Central European countries; however, it may reduce the topsoil organic carbon pools in the converted forest stands.
Taking the advantage of Landau transformation, we restore the concentration profile of drug in a polymer matrix, the rate of release of drug from the polymer matrix, and the temporal variation of location of the moving boundary taking the external mass transfer resistance into account.
Therefore, therapeutic interventions that can counter these oncogenic transformations and/or restore immunovisibility would be of value.
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