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Discovery of second site mutations that restore the activity of some of these mutants has provided clues for the restoration of activity by the use of small molecules.
To further dissect the backbone requirements for the tracrRNA and determine if any DNA residues could be tolerated, regions of the tracrHyb were reverted to RNA and tested for restoration of activity.
The FVa-dependent restoration of activity is a key aspect to their efficacy and also contributes to localizing the variants to the site of vascular injury.
Restoration of activity is greatly reduced by mutation of the two CRE motifs.
Broadly speaking, any mechanism that yields the restoration of activity in the PI3K, MAPK, AKT or mTORC1 pathways, or increased activity of the ER pathway, will restore the proliferation-inducing and/or apoptosis-opposing effects of these pathways, and will yield a decrease in the effectiveness of PI3K inhibitors.
The Ebola virus VP30 protein, which is required for replication/transcription by the RdRp, could be complemented in trans for restoration of activity in Ebola-VLP-infected target cells [60].
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Although geriatric rehabilitation (GR) is beneficial for restoration of activities and participation after hospitalization of vulnerable older persons, little is known about the optimal organization of care of these postacute facilities.
A subjective inquiry was directed to both the parents and the paediatric patients with respect to their impressions of the full restoration of activities of daily living and participation in children's games generally.
We predicted that if loss of Sod1 activity was the source of the ROS, restoration of that activity by overexpression would result in ROS levels similar to zinc-replete cells.
Reconstitution of complement activation with rFicolin-3 allowed full restoration of complement activity.
In mice lacking telomerase activity, progressively shortened telomeres is associated with premature aging [2], while restoration of telomerase activity rescues this phenotype [3].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com