"We decided to put Gyllene Tider to rest... until further notice".
🚨🚨🚨WE ARE ON HIGH ALERT UNTIL FURTHER NOTICE 🚨🚨🚨 https://t.co/jQSqacc9fc.
Like any good scientist, I could not rest until I investigated further.
The management of the Montreal Symphony Orchestra announced that it was canceling the rest of the season until further notice after its musicians said that they would strike on Monday.
The homogenized suspension was centrifuged at 2,500 g for 5 min; 250 μL of the supernatant was used for RNA extraction by using TRIZOL LS Reagent (Invitrogen Life Technologies, Carlsbad, CA, USA), and the rest was stored at –70°C until further use.
"Needing complete rest, Steve will not be contactable until further notice," the statement said.
So now, until further unrest, we'll rest in peace.
The left ventricle (LV) plus septum were separated from the rest of the heart and stored at −80 °C until further analysis.
The rest of the EBN was stored at -80°C until further analysis, while the boiled sample was kept in a shaking incubator LSI-30166, Daihan Lab tech Co. Ltd, Korea) at 55°C and 50 rpm for 3 h.
The rest of the material was immediately frozen and stored until further analysis.
An aliquot was used to determine soluble protein concentration and the rest of the cytosolic extract was frozen at -20°C until further assay.
