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The conical shape of the container allows the sample to rest stably with a minimum amount of medium surrounding it.
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The ship was in delicate condition, he told me, but resting stably.
Most recently, the Concordia has been winterized and rests stably afloat off the Italian coast in what the parbuckling team calls the "refloating" phase.
If your pumpkin already has a relatively flat bottom and rests stably, you can skip this step.
However, re-analysis where FARP1 was included with the rest of the five stably expressed genes (with n = 35) did not change the final results (data not shown).
The REST/KD-50 ES cells were then used to create REST-null ES cells by stably expressing a Rest shRNA (5'-GTGTAATCTACAATACCAT-3') in the presence of puromycin (2 µg/ml) using a pSuper-Rest-shRNA vector [43].
Epifluorescence images of α-SNAP and scr RNAi treated, resting HEK 293 cells stably expressing Orai1-YFP (A ) or YFP-Stim1 (B ).
TRIM does not have such amino acids in its transmembrane region [ 16] and thus might associate less stably with the rest of the αβTCR.
U.S. Eastern Standard Time touchdown, European scientists and engineers struggled to make sense of how stably the lander was resting on comet 67P/Churyumov-Gerasimenko. Radio connections between the lander and the orbiter were tenuous, solar power generation was fluctuating, and the lander was not anchored by two harpoons that were supposed to have fired on touchdown.
We find that after this transient replacement, Cse4 remains stably associated with centromeres for the rest of the cell cycle, without additional Cse4 deposition in anaphase.
To test this idea we performed a mass spectrometric analysis of REST complexes using a mouse ESC line that stably expressed both the biotin conjugating enzyme, BirA (Kim et al., 2009), and REST tagged with a biotin acceptor sequence.
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