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The responsiveness of the cell lines MDA-kb2 (responsive for testosterone) and T47D-KBluc (responsive for estradiol) for hormone treatment was measured by a luciferase assay as both cell lines contain a hormone responsive element upstream of luciferase reporter gene [50], [51].
This is because there is a significant degree of variability at a biological level but also at a technical one; even minor changes in the degree of expression, disturbance on drug addition, and responsiveness of the cell can play a role in the extent of cellular response [ 27, 40].
Then the electrode was withdrawn slowly from the cell interior and the responsiveness of the cell was checked again extracellularly.
The injection was performed by passing negative current pulses, 1.0 nA in a 100 ms on/200 ms off cycle, for a period about 10 min. During current injection, the normal responsiveness of the cell was continuously monitored using a visual stimulus.
There are differences in the responsiveness of the cell lines which were chosen to represent either breast or bladder cancer.
We found a similar proliferation ratio for both polymorphic forms of GMRα, indicating that the polymorphism does not affect responsiveness of the cell to GM-CSF.
Similar(51)
Therefore, expression of different SpTPC isoforms affects responsiveness of the cells rather than amplitude of the response.
Therefore inhibition of one of these axes will not prevent pro-metastatic responsiveness of the cells to other axes.
Continued responsiveness of the cells to prostaglandin F2α stimulation after 6 hours of oxytocin pretreatment indicated that postreceptor signaling pathways were maintained, which indicates that the oxytocin receptor likely is involved in the mechanism of myocyte desensitization to oxytocin stimulation.
The responsiveness of the cells was also tested with different naturalistic stimulus sequences (data not shown).
The level of responsiveness of the cells was found to be unaltered by exposure to the transfection reagent (Supplemental Figure S1).
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