Similarly, transfection of ActR-IIB into ATDC-5 cells did not alter GDF-5 responsiveness (Additional file 5: Figure S4B).
In addition, GO analysis revealed nine genes involved in ABA signaling and ABA responsiveness (Additional file 5: Table S2).
Here we found significant association between the presence of AuxRE/Y-patch composite element in gene upstream regions and its auxin responsiveness (Additional file 8).
This implies that ligand identity, rather than cell type or downstream pathway, is the main determinant of low-dose responsiveness (Additional file 6: Figure S2G).
A similar result was obtained using average fold-change ≥ |2| (treatment versus control) as a criterion to determine gene responsiveness (Additional Files 17 and 18).
The researchers also explored the perceived health providers' responsiveness (refer to Additional file 1).
Promoter analysis of ADP-glucose pyrophosphorylase and vacuolar invertase genes indicated the presence of cis-regulatory elements that supported their responsiveness to defoliation (Additional files 6 7 8).
Full gene lists grouped by responsiveness are available in Additional file 2. Fig 1A and 1B show the number of genes and their overlapping responses.
All the results were similar when using a 15% relative or a 300 ml/min/m2 absolute cutoff for volume expansion-induced increase in CO to define fluid responsiveness (Table 3 and Additional file 1, Figures S1 and S2).
