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Our results indicate that not only was a trend conserved for the dose responsive effects, but also a time –responsive up and down-regulated trend among these compounds was conserved, implicating that a common underlying mechanism may be shared by these compounds.
We used the PCR-based SSH approach to identify MJ responsive (up- and down-regulated) transcripts that precede paclitaxel accumulation in the paclitaxel accumulating T. cuspidata P991 cell line.
The modified electrode is responsive up to a pH of 7. The stability of the modified electrode during continuous electrochemical cycling is poor, due to leaching of the dopant from the PEDOT film.
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For the set of core cold responsive up-regulated genes, tolerant cultivars showed low expression levels both at harvest and during cold storage conditions, whereas sensitive cultivars showed increased expression in the cold.
Interestingly, unlike the known Wnt targets in C. elegans, only one of the 104 BAR-1 responsive up-regulated genes encodes a transcription factor (sptf-2 ); the category of nucleic-acid binding proteins was under-represented (Table S3).
The second condition was that the genes had to be reported as cold responsive (up-regulated in any pathway) at least twice, either in genome-wide expression studies [ 20, 21, 126- 128], in their annotation description, or in the aforementioned functional studies.
Computational analysis of the genome-wide distribution of the predicted AuxREs and their impact on auxin responsive gene expression allowed us to conclude that: (1) AuxREs are enriched around the transcription start site with the maximum density in 5'UTR; (2) AuxREs mediate auxin responsive up-regulation, not down-regulation.
MJ-responsive up-regulation of most genes, however, did not persist into the latest time point 5 day.
Although genotype-specific differences were present, the observed similar expression patterns indicate a possible trichothecene-responsive up-regulation for TaMDR1 as well.
The ratio of AtfA-dependent, up-regulated genes and stress-responsive, up-regulated genes was also high in the case of l-H2O2 and NaCl treatments (Table 5).
The ABRE-related motif is a cis-element identified in the upstream region of 162 Ca2+-responsive up-regulated genes [ 48].
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