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Detailed pathologic studies have also demonstrated surprisingly widespread, non-motor pathology in some ALS patients, typically in the form of ubiquinated cytoplasmic inclusions that are immunoreactive for transactivating responsive sequence (TAR) DNA-binding protein 43 kDa, or TDP-43 [ 3].
Such fine-tuning in the chromatin structure precisely in STAT responsive sequence might recognize the non-histone proteins for the transcriptional activation of cdk-inhibitor p21 WAF1 gene [ 15, 16, 50].
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As shown in Figure 5 of these BZLF-1 responsive sequences can further be localized within a 200 base region immediately adjacent to the BALF-2 ORF [48].
GATA-3 responsive sequences have been identified in the 5' flanking regulatory area of HSD1 [ 32].
By using combination of microarray and SBS sequencing, a total of 125 putative long npcRNAs were identified in wheat leaves using strict criteria across a collection of more than 9700 powdery mildew and 6500 heat stress responsive sequences.
In this article, we proceed to a fine study of the transcriptome and report the location of 281 RYMV stress responsive sequences (RSR sequences identified by cDNA-AFLP).
To test whether these sequences are functional, we cloned the 3′UTR of both human and mouse genes, or the putative miR-33 responsive sequences, immediately downstream of a luciferase reporter.
A duplex DNA (P1-P2 hybrid) containing the methylation-responsive sequence is ingeniously designed.
Two activin-responsive promoter constructs were used to assess inhibin bioactivity; an ovine FSHβ promoter (oFSHβ-lux), and a construct containing three copies of the activin-responsive sequence from the GnRHR promoter (3XpGRAS-PRL-lux).
Analysis of various TSP1 promoter mutant constructs showed that a sequence located −144/−137 up-stream of the transcriptional initiation site, related to the consensus E2F-responsive sequence, is necessary for the activation.
Another example is the dehydration and senescence-responsive sequence CACGTAAGT from the SAG113 promoter (37).
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