The tetracycline-responsive master clones are potentially powerful tools for studying the function of various genes in postnatal myogenesis.
Since the seminal discovery of HIF-1α as a hypoxia-responsive master regulator of numerous genes and transcription factors, several groups have reported a novel mechanism whereby hypoxia mediates stabilization of HIF-1α.
More complicated feed forward loops (FFL) involve three factors: a signal-responsive master regulator TF, an intermediate TF controlled by the master TF and a jointly regulated gene under the control of both the master and intermediate TFs [ 12].
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Our genome-wide gene expression profiling identified notable upregulation of a series of p53-responsive genes, including a master regulator of growth arrest and cellular senescence, p21.
These generally include a P-responsive histidine kinase phoR, a master regulator phoB, P-specific ABC transporters, and alkaline phosphatase phoA, as well as P-metabolism genes.
Thus it is rational that some of the stress-responsive regulatory networks and their master regulators are present in different organisms, and work under similar mechanisms as observed in plants to promote acclimation to the stressing conditions.
CIAT 899 and PRF 81 have genes for RirA that has replaced Fur as the master regulator of iron-responsive genes in the Rhizobiaceae; and Irr, a second, minor-acting Fe-responsive regulator present only in the Rhizobiales and Rhodobacterales [ 121].
But he has been different from all the other aspiring masters in that he has taught nothing so insistently as the need to dissolve our illusions about masters, and to be responsive to more moderate, subtle and humane sources of authority.
Mouse myoblasts from a long-term culture (i28 cells) were transfected initially to generate and characterize two stable master clones expressing tetracycline-responsive transactivator protein tTA.
Pgc1a and the sterol-responsive element binding protein 1 (Srebp1) are master regulators of lipid biosynthesis and breakdown (22, 23).
