These processes amount to a metal ion responsive folding and assembly process that is reminiscent of RNA folding processes.
The Ta.22614.1.S1_at gene was selected for qPCR analysis because of its relativelyhigh and FHB-responsive fold change (9.49 up) at 72 hai (Additional file 3).
Other genes showed differences in expression levels and were less responsive (smaller fold changes) in the GWI cases compared to controls, such as GZMB, CCL4 and FCG3A (alias CD16) (additional file 4).
The most upstream promoter is only slightly TNF-α-responsive (1.3-fold increase, P < 0.01), while the alternative promoter is more responsive (3.6-fold, P < 0.001).
Luciferase reporter assays indicated modest induction of transcriptional activity of β-catenin (2.05-fold (Panc1) and 1.92-fold (MiaPaCa)) and NF- κB responsive promoter (2.98-fold (Panc1) and 2.26-fold (MiaPaCa)) in CXCL12-treated cells.
On the other hand, the lower representation of de novo FA synthesis observed in the H group was validated by the higher expression of the thyroid hormone responsive (THRSP) gene (fold difference = 3.46, p-value = 3.36 × 10-06).
The criteria to consider a miRNA to be drought-responsive are |log2 fold change| ≥ 2 and p-value ≤ 0.05 [ 76].
E2 dose-dependent cAMP production, although biphasic in both cell types, was responsive to 50-fold higher E2 concentrations in mERhigh cells.
Finally, Log 2 ratio data from the microarray data was analyzed to determine which genes in the genome were greater then 1.5-fold responsive in any pair of replicate experiments in this meta data set.
The PAR-1a (photoassimilate-responsive) protein (+22 fold in INF vs CON) is a serine/threonine kinase with diverse phosphorylation targets and has been reported to be induced by infection with potato virus Y [ 60, 61].
It is possible to show analytically (see Additional file 1) that iMSLs are responsive to input fold-change if three conditions are satisfied: Strong repression: s/ h ≫ 1 ⇒ k p (s) ≈ k p h/ s(condition for perfect adaptation), Almost linear promoter activation k r (q) ≈ q k r / h r, Fast mRNA dynamics (short mRNA half-life with respect to miRNA and protein ones): r(t)→ r ss.
