This novel GSM pharmacology is dose-responsive, driven by the plasma free-fraction of the compound and is capable of reducing both Aβ42 and Aβ38 levels in APP over-expressing mice.
He wanted to create a narrative structure that would "relate to deep and responsive drives, needs and fantasies in the American audience" and to "the preoccupation with guilt and salvation which has been called the American Theme".
The VHL-deficient 786-O cells were transiently transfected with vector, vector expressing wild-type VHL or its mutants along with HIFα responsive element driven luciferase reporter.
The effect of WOX1 on responsive promoters driven by transcription factors was determined using the following reporter constructs (Stratagene), as described [31], [32]: (a) Luciferase reporter gene linked to tandem repeats (4X) of CRE and AP-1, respectively; (b) A Gal4 reporter system specific for transactivational activity of CREB, c-Jun and c-Elk-1, respectively.
To examine Noggin activity against BMP (bone morphogenetic protein), a BRE (BMP responsive element) driven luciferase assay was conducted to determine if addition of Noggin could decrease BMP activity.
Importantly, we present a proof-of-concept experiment to synthesize polySBAA nanogels, which show a switchable property between antifouling and pH-responsive functions driven by acid base conditions, while still maintaining high stability in undiluted fetal bovine serum and minimal toxicity to cultured cells.
Because a heat-responsive promoter drives the AXR3NT-GUS construct, we tested whether the lack of AXR3NT-GUS activity in ibr5 could be explained by a reduced transcriptional response to heat in the mutant.
The cell lines were cotransfected with an AR expression vector and an androgen-responsive luciferase gene driven by the mouse mammary tumor virus (MMTV-luciferase) promoter.
The membrane of the proposed mini-eggs is composed of a Ca-alginate/protamine composite layer encapsulated by a silica surface layer, in which the former provides the pH-responsive switching function driven by electrostatic interactions between Ca-alginate networks and protamine molecules, and the latter offers protection for the former to guarantee the stability of pH-responsive performance.
Expression of antioxidant-responsive-element (ARE-) driven genes and enzymes is directed by Nrf2 cap'n'collar bZIP transcription factors.
Interestingly, Rh1 does not enhance the transactivation of glucocorticoid-responsive elements (GRE) driven genes - gluconeogenic enzyme glucose-6-phosphatase (G6P) and phosphoenolpyruvate carboxykinasee phosphatase (PEPCK) in primary mouse hepatocytes, a mechanism partly held accountable for the metabolic side-effects.
