The T22 reporter cells are stably transfected with a p53-responsive reporter construct, pRGCd-Fos-lacZ, as described elsewhere (Hupp et al, 1995; Bottger et al, 1997).
The fraction of CD4+ and CD8+ T cells responsive to CMV pp65 or IE-1 was determined by CFC, as described elsewhere [ 8].
Now, tongue as described.
De Boer et al. (unpublished data) constructed cDNA libraries enriched for stress responsive genes as described by [ 24].
A total of 1411 genes were identified as being differentially expressed (FC >2, FDR <0.05), of which 247 were Ph-responsive genes as described above.
However, we have not ruled out that, once upregulated by RA signalling, β-catenin might synergize with RARs to activate RARE-responsive promoters, as described previously [ 78].
To prepare transcript profiles of field plants we used a cDNA microarray containing 3628 unique sequences derived from libraries enriched in stress-responsive cDNAs as described in Wong et al. [ 19].
The reporter gene vector was constructed with the human β-globin DNA sequence placed under the control of a Tet-responsive promoter, as described previously (Janicki et al., 2004; Darzacq et al., 2007).
A stable TREX 293 cell line expressing the tet repressor and human WNT1 under the control of a tet responsive promoter was cultured as described above and transduced with recombinant virus coding for a (12X TOPFlash luciferase reporter and a constitutive renilla luciferase.
