DNA-binding activity is associated with its C-terminal DNA-binding domain (DBD), while the N-terminal transactivation domain (TAD) is responsible for replication and transactivation functions.
The West Nile virus (WNV) genome contains a single RNA-dependent RNA polymerase (RdRp) gene, which is responsible for replication of the viral genome and, as such, is an important target for antiviral therapy.
Polymerases responsible for replication have properties that are different from those responsible for repair.
The instructions responsible for replication are the ones in positions with 0 mutations.
Polymerase gamma (Pol γ) has a similar error rate (10−5 10−6) and is responsible for replication of the mitochondrial genome (McCulloch and Kunkel 2008).
ORF2 encodes a protein of approximately 95 kDa with critical endonuclease and reverse transcriptase activities as found in mammalian L1s, and is responsible for replication of the element.
While DNA-A encodes proteins and regulatory elements responsible for replication, encapsidation functions and control of gene expression, DNA-B encodes proteins enabling viral movement [ 2].
It is thus possible that the downstream origin (which positions (CAG n in the lagging strand template) is responsible for replication and expansion of the TNR in the transgenic DM328 cells.
We also demonstrate that TWINKLE is essential for nascent H-strand synthesis in the D-loop, thus showing that there is no separate DNA helicase responsible for replication of this region.
Although the assay used did not allow clearly defining the mechanism, the study suggests that low efficiency of origins located within CFS, with respect to those in nonfragile regions, may be responsible for replication perturbation along fragile sites.
In addition, orc/cdc6 genes were found on most of the extrachromosomal elements (Table 1), suggesting that the orc/cdc6-associated replication origins are responsible for replication initiation on most of these replicons.
