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Manmade protein design is founded on the concept that a protein with minimal evolutionary complexity is a viable scaffold for incorporating simple engineering elements responsible for function in natural proteins and enzymes.
TrpAb is the glutamine-binding subunit of anthranilate synthase responsible for function of the TrpAa/TrpAb complex as an amidotransferase.
Berthier et al. (1986) hypothesized that the initiator for the transcript responsible for function of these six genes in the Drosophila mitochondrial genome is in either the nad6 or cytb gene.
The Ka/Ks analysis demonstrated that Ka/Ks ratios for all the paralogous OsPTR genes were always less than one, most of them even close to zero, suggesting that purifying selection might be largely responsible for function maintenance of this family (Table 1).
These results open two alternative hypotheses: 1) just the presence of ciaB is not enough to warrant a successful invasion, considering that both C. showae and C. hominis presented a reduced repertory of virulence genes; or 2) sequence variation at amino acid level was responsible for function switching and/or specialization of this gene.
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With an education that's spotty at best, and no family or other support systems in place, you're told that you're now an adult and responsible for functioning in the world on your own.
This suggests that the ferlin C-terminus is perhaps responsible for functions more fundamental, than specialized.
Moreover, it showed extensive interactions between AA-NAT and 14-3-3 14-3-3 14-3-3h may be resproteine for functioning of intricate regulatory mechanisms.
In contrast AAs responsible for functions like heterodimerisation or signalling were more conserved, presumably because the interacting molecules require conserved AA binding sites.
These data suggest the ferlin N-terminus could be responsible for functions that are more species specific, and/or ortholog specific, rather than unified across the ferlin family.
(1) In more basal lineages BMP signaling is responsible for functions that are performed by Toll signaling in more derived lineages.
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