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That CD8+ T cells were responsible for the observed responses was confirmed for EZH2 by both IFN- γ capture assays and tetramer staining using an HLA-A0201-restricted HLA-A0201-restricted HLA-A0201-restrictedtope.
The apoptotic nature of these macrophage-induced cell death responses was confirmed by the blockade of cytotoxic activity in the presence of the pan-caspase inhibitor, zVAD (Fig. 5b, white bars).
That pmif RNA immunization is associated with an improvement in the host Tfh and B cell responses was confirmed by serum antibody titers against Plasmodium blood- and liver-stage (CSP) antigens, and a 6-8-fold higher titer of total IgG, was observed against blood-stage and liver-stage antigen, respectively, in the pmif RNA versus control RNA immunized groups (Fig. 6f, g).
The role of dendritic cell-derived thrombospondin-1 in regulating secondary allergic T cell responses was confirmed in vivo, as local transfer of thrombospondin-1-sufficient dendritic cells to the ocular mucosa of thrombospondin-1 null hosts prevented the development of augmented secondary T cell responses and heightened allergic eye disease clinical responses.
Oligofunctionality of the vaccine-induced CD8 T cell responses was confirmed by a multicolour flow cytometry assessing production of IFN-γ, TNF-α and CD107a (Fig. 4 B).
This induction of adult-like neonatal responses was confirmed by assessing the cytokine frequency and production of IFN-γ IL-2, TNF-α and IL-17 (Table 1).
Similar(46)
The effect of Aer solids on the material characteristics and the relationship between void distribution and material responses are confirmed from the obtained results.
Where possible, responses were confirmed through visual observation.
Positive responses were confirmed by ICS assay.
However, in contrast to Addo et al [30] no nef specific responses were confirmed in our assays.
For the majority of subjects, Env pool-specific responses were confirmed at the individual peptide level (n = 61).
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