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INF-γ levels ≥ 0.35 international units (IU /ml (TB antigen response minus nil response) were considered a positive test.
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Pai et al. reported 24% reversions on serial testing in health care workers with 78% of them occurring with TB minus nil responses <1.0 IU/ml [ 13].
QFT-IT was indeterminate in 19 cases (16%), 14 were due to low mitogen responses and 5 due to a high nil response.
Percentage responses, i.e. increase, decrease or remain in the same value, are expresses as a balance, i.e. percentage of favorable responses minus unfavorable responses.
The QFT-GIT was performed according to the manufacturer's instructions that consider a result positive if the IFN-γ response of TB antigen minus Nil was ≥ 0.35 IU/ml [ 29].
QFT was performed and interpreted according to the manufacturer's instructions, with an interferon-γ response for the tuberculosis antigen tube minus Nil of ≥0.35 IU/ml defined as a positive result.
For those who did not have a consistent QFT response (TB antigen response corrected for Nil control) > 1.0 IU/mL in the second testing, QFT was repeated for the third time.
The opportunity to respond/refute is yours- nil-response would equate to arrogance, uncertainty or worst of all for your employers - insouciance!
In the present study only one assay had a high nil control response as defined by the manufacturer's latest guidelines for test interpretation that allow a significantly higher background (nil control) response of up to 8 IU/ml.
But the response, the impulse response is now 1/2 minus 1/2.
Our data, as well as those of others [ 13, 27], suggest that some allowance should be provided in the interpretation of low IFN-gamma responses (TB antigen response corrected for Nil control values 0.35-1.0 IU/mL) where reversions are particularly common.
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