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Replicative senescence is triggered by DNA damage response foci associated with telomeres.
Loss of arginine methylation in TRF2 induces DNA-damage response foci at telomeres and triggers cellular senescence [ 29].
For example, 8 consistently occurring response foci to either low- or high-frequency stimuli were identified (Talavage et al. 2000).
The inhibition of tumor cells by pretreatment with T-oligo was associated with increased induction of senescence and apoptosis of irradiated tumor cells and reduced clonogenesis, presumably due to the observed increased formation and/or delayed resolution of DNA damage response foci.
To test for this association, we pulsed E13.5 Cre+, Rest GTi/GTi and control embryos for 30 min with BrdU, to label cells in S phase, and co-stained BrdU+ progenitors and neurons with antibodies to 53BP1 and clCasp3 for damage response foci and apoptosis, respectively.
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CEO of Professional Science Editing for Scientists @ prosciediting.com