Sentence examples for response elements from the from inspiring English sources

Because of the absence of estrogen or progesterone response elements from the plasminogen activator inhibitor 1 gene promoter, I posited that epidermal growth factor mediates these steroid effects and that expression of epidermal growth factor receptor in human endometrial stromal cells is under ovarian steroid control.

NF-κ B-RE-luc is a light producing animal model that carries a transgene containing 3 NF-κB response elements from the Igκ light chain promoter and modified firefly luciferase cDNA (Promega pGL-3) [ 23].

We conclude that in vivo, the BMP and activin A sentinels reveal overlapping and distinct domains of signaling, and that the −228 sentinel is more useful for activin A. To develop live signaling sentinels for the Wnt pathway, we employed the Wnt response elements from the SuperTOP-Flash construct, which uses a luciferase reporter assay with lysed cells (Veeman et al., 2003).

The plasmid pAMS366 contains a tandem of four CDREs (calcineurin-dependent response elements) from the GSC2/ FKS2 gene fused to the lacZ reporter [ 33]. pPHO84-LacZ contains a PHO84-LacZ reporter fusion as described in [ 19]. pKC201 contains the ENA1 promoter fused to LacZ [ 34, 35].

Also, GATA-induced gene expression could be reduced or ablated by the removal of putative GATA response elements from these control plasmids.

Y220C, for instance, still transactivates the most sensitive wt p53 response element (from the promoter of the p21 gene—whereaswhereas other response elements are not activated (38).

This approach improves specificity of retinoid detection compared with alternative approaches, such as using the retinoid response element from the RARB promoter, which may respond nonspecifically to Rara/Rxra and Rarg/Rxra heterodimers, and Rxra/Rxra homodimers.

As positive controls we used iRFP expression constructs containing p53 response element encoding repeats of a known p53-binding sequence (p53RE), the p53-binding site of p21 (Wand) and a p63 response element from the skin-specific promoter of bullous pemphigoid antigen 1 (BPAG1).

A luciferase reporter gene plasmid (pMWM-30, MW Madsen, unpublished; 1 μg) with four copies of a DR3-type vitamin D response element from the rat atrial natriuretic factor promoter [ 31], an expression vector for VDR (pSG5-VDR; 0.5 μg) and the internal transfection control CMV-LacZ (0.1 μg) were cotransfected in triplicate using Fugene 6 (Roche Diagnostics Scandinavia AB, Bromma, Sweden).

Homogeneity of steroid HRE is analyzed and a reliable training dataset is constructed through extensive searching for experimentally validated response elements from more than 150 literature sources.

Prior studies have isolated individual response elements from complex regulatory sequences and tested them as multimers (Schirm et al., 1987), so that the element would report the appearance of an individual signal when linked to a convenient marker.