The influences of many participating precursors and cofactors on lysine synthesis flux were also investigated through the calculation of response coefficients for individual parameters.
The response coefficients calculated using the AS/NZS (2002) provisions were generally a good match for the interior region only, however they significantly underestimated the wind tunnel response coefficients for the end bays.
where h1,m(k) and h2,m(k) respectively denote the normalized complex channel impulse response coefficients for the k th bit between the 1st and 2nd transmit antennas and the m th receive antenna; e m is a Gaussian noise with zero mean and variance NN0/2.
Additionally, the response coefficients for stromal glucose to the glucose transporter kcat and KM are substantial, whereas those for cytosolic glucose are not, consistent with a kinetic bottleneck due to transport.
The β-amylase-associated response coefficients for cytosolic glucose, arabinogalactan, and glucosylated arabinogalactan have their second largest values for any enzyme, despite these metabolites being the most distant variable metabolites from chloroplastic maltose in the reaction network.
Ainscow and Brand attributed the high internal response coefficients for the flux of pyruvate through lactate dehydrogenase in rat hepatocytes to the fact that lactate dehydrogenase was working close to equilibrium [ 32].
By quantifying these effects, the partial internal response coefficients allow the assessment of their importance in restoring the steady state [ 32, 62], i.e. (13) According to the connectivity theorem the sum of the partial internal response coefficients for each intermediate is -1.
The internal response coefficients for the system under discussion are listed in Additional file 4. The cellular responses to elevated pyruvate level were in line with previous findings in rat hepatocytes, i.e. elevated pyruvate levels were mainly counteracted by an increased flux through the lactate dehydrogenase enzyme (r18) [ 32].
The response coefficient of greatest magnitude for glucosylated arabinogalactan corresponds to the equilibrium constant for glucosyl transfer, emphasizing the locality of this metabolite's response.
