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We have demonstrated how these signaling pathways with specific sensitivity towards expression of FLO11 are integrated to respond under nitrogen starvation.
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However, dryland systems often respond to nitrogen fertilization as well as increased water availability.
Their transcription levels respond to nitrogen starvation, suggesting that the prochlorosins are functional.
Because the segregating generations were grown under moderate and fluctuating levels of nitrogen, it is to be expected that genotypes with good nitrogen use efficiency will be selected that can also respond to added nitrogen.
Well known two component regulators respond to osmolarity, nitrogen and oxygen limitation [44], [45].
Hence, as expected, CAP contents in pepper pods respond to external nitrogen availability.
In fact, only 17%% of the genes reported by Williams et al. responded to nitrogen limitation in continuous culture, including 70 genes that were predicted to be under control of GlnR (Fig. 2a) [ 20].
GmAlaAT also responded to nitrogen availability in the solution during waterlogging.
Even more poorly described are the pathways sensing and responding to nitrogen levels.
In general, we show that these global signaling pathways respond with specific sensitivity to regulate the expression of FLO11 under nitrogen limitation.
In this model system, the GATA-factor AreA responds to intracellular glutamine (Gln) concentrations and is active under nitrogen limiting, low Gln conditions.
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