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We utilize genetic and genomic tools to uncover how different types of retinal cells, including retinal neurons, glia and the vasculature, respond to developmental cues and disease insults at the epigenomic and transcriptional levels, and how they interact and collectively contribute to the integrity of the retina.
We demonstrated that emNCSCs give rise in vitro to all NC lineages, are multipotent on clonal level, and appropriately respond to developmental factors.
These "bivalent" genes were shown to correspond largely to genes that were poised to respond to developmental signals [ 80– 80].
We also developed a single cell assay to quantify PKD2 activation as thymocytes respond to developmental stimuli or expression of α/β TCR complexes in vivo.
The regulation of gene expression in the cell is driven by fine-tuned molecular mechanisms that respond to developmental and environmental cues.
We also developed a single cell assay to quantify PKD2 activation as T-cells respond to developmental stimuli or engagement of α/β TCR complexes in vivo.
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In all these cases, emotional abuse in childhood (Stein et al., 2008) or a generally adverse childrearing environment (Retz et al., 2008), it proved to be those individuals carrying short alleles who responded to developmental or concurrent experiences in a for-better-and-for-worse manner, depending on the nature of the experience in question.
All but those wing traits presumably involved in mate choice responded to developmental temperature and, of those, all but the wing traits not exposed to predators responded to hormone manipulations.
Although accumulating evidences indicate that AS is developmentally regulated, how AS responds to developmental cues is not well understood.
While about 25% of DEGs responding to developmental time were shared between locations (n = 108), a large number of genes were differentially regulated in Mexico (n = 245) or Florida (n = 105) alone, providing a major component of the clear differentiation between locations observed in the hierarchical clustering (Fig. 3; left panel).
In the present study, particular attention was focused on the detailed temporal profiling of miRNAs responding to developmental stage transitions of silkworms.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com