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The targeting of PaMT1 into the mitochondrial matrix of P. anserina was found to lead to a doubling of the lifespan which partially can be attributed to a reduced generation of ROS in mitochondria respiring via the alternative pathway [6].
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Several long-lived mutants are known which predominantly or exclusively respire via AOX.
Normally, strains respire via a cytochrome-c oxidase (COX, complex IV) dependent respiratory chain.
Oxygen-consumption revealed a reduction by 30% of KCN-sensitive COX-dependent respiration and a concomitant induction of SHAM-sensitive respiration via AOX in the transformants (Fig. 5A), compared to the wild-type which almost exclusively respires via COX.
It has been proposed that one function of this 'NO-cytochrome c oxidase signalling' interaction is to regulate O2 gradients within cells and tissues via limiting O2 consumption in actively respiring mitochondria [ 43].
In the coupling assay, succinate (a FADH2-linked substrate of complex II) was used with rotenone to allow the mitochondria respire via complex II as succinate without rotenone would form oxaloacetate from malate by the action of malate dehydrogenase.
A very low proportion of total bacteria was actively respiring (ca. 16%).
A high proportion of the total bacteria (ca. 83%) was actively respiring.
She straightens slowly, respiring deeply.
I require oxygen to continue respiring.
Only coupled, respiring mitochondria will take up this fluorescent dye.
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CEO of Professional Science Editing for Scientists @ prosciediting.com