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COX5A deletion (Δ cox5A) results in a lack of respiratory competence [ 24] and absence of C cO. Respiration-competent mutants of such a Δ cox5A strain had mutations in ROX1, a repressor of COX5B, that resulted in the enhanced expression of COX5B [ 24].
Cells expressing Mdj1∆D also showed a decrease in respiratory competence.
Cells expressing the ΔC allele lost respiratory competence with kinetics similar to that observed for Mdj1H89Q (compare Fig. 4B with Fig. 3B, Suppl. Fig. S4).
To test whether loss of respiratory competence upon depletion of Mdj1 actually reflected changes in mtDNA, we characterized the mtDNA present in Mdj1-depleted cells.
A subtle difference in how fast hypoxic cultures depleted the available oxygen led the group to explore the respiratory competence of the mutant more closely.
Next we measured the steady state levels of respiratory complex proteins to determine whether the partial growth defect suppression truly reflected an improvement in respiratory competence.
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The plasmid pRS415-COX5A COX5AiningeneX5A gene under the control of its promoter and terminator was a gift from D. Winge (University of Utah, Salt Lake City, UT, U.S.A .. Respiratory growth competence was checked on YPG medium (1% yeast extract, 2% peptone, 2% glycerol and 2% agar).
In Senegal and Bolivia, training provided improved CHW theoretical competences in respiratory diseases management, but practical competences were not assessed [ 12, 13].
Failure to wean has been attributed to an imbalance between the load faced by the respiratory muscles and their neuromuscular competence.
It also provides leads for new investigations into B12 metabolism in bacteria and other aspects of protein-ligand interaction in competence, cell-surface biochemistry, and respiratory electron transfer.
To develop and validate a self-evaluation tool (questionnaire) to identify perceived competence and confidence felt by physiotherapists undertaking respiratory on-call duty.
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