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These genes, and others from the TCA branched pathway, displayed the same pattern of transcription as those involved in respiration (data not shown).
Addition of excess cytochrome C to the chamber in the presence of pyruvate and ADP did not increase respiration (data not shown); indicating that during isolation outer mitochondrial membrane integrity was retained.
Basal respiration (Fig. 2A), ATP-linked respiration (Fig. 2B), basal glycolytic rate (Fig. 2E), proton leak and non-mitochondrial respiration (data not shown) were similar in the 3 groups.
Further, some respiration cases (according to the classification criteria used in the study and based on the information in the VCR) were designated "without diagnosis" (in system whole body) in the CCR when the VCR information indicated respiration (data not shown).
Similar(56)
The addition of NADH during state 3 measurements had no effect on the respiration rate (data not shown), indicating excellent mitochondrial membrane integrity.
The latter effect was confirmed based on uncoupled mitochondrial respiration rate measurements (data not shown).
In concentration higher than 60µM oleate did impair both respiration and heat production (data not shown) regardless of the presence of Ca2+ in the medium.
To elucidate a potential quantitative correlation between enzyme levels and degree of respiration, we plotted the measured abundance for each enzyme against the corresponding degree of respiration and calculated the correlation factor (data not shown).
We confirmed that none of these mutants could grow on glycerol where respiration is the only energy source (data not shown).
This acute metabolic perturbation did not have any significant effect on mitochondrial respiration in any of the subjects (data not shown).
In contrast to our previous studies with lines with decreased activities of other enzymes involved in organic acid metabolism, we observed no major differences in either assimilation rate or dark respiration in the ICDH antisense lines (data not shown).
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CEO of Professional Science Editing for Scientists @ prosciediting.com