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Under normal capacitation, spermatozoa respired at a rate that constituted less than 40%% of total respiration capacity (Fig. 6A).
Seven environments from cultivation to late abandonment, with the same soil type classified as Lithic Xerorthent, were tested to ascertain the respiration capacity according to the current use and cover, and to establish the ability to preserve and eventually increase the organic matter pools after abandonment.
Either by fine-tuning the respiration capacity or by using a dual-phase fermentation approach involving a switch from fully respiratory to non-respiratory conditions, we obtained 361 mM (32 g/L) R-BDO with a yield of 81% or 365 mM (33 g/L) with a yield of 82%, respectively.
This is believed to be a measure of "spare respiration capacity (sRC)" [47], [48].
Liver mitochondria appear to have a lower capacity to produce ATP because of lower state 3 respiration capacity and higher proton leak through the inner mitochondrial membrane.
This enables survival despite diminished respiration capacity.
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For example, the presence of gain of function mutations affecting biofilm formation, nanowire formation, cell membrane electron transfer, metabolic and respiration capacities, regulatory components for these functions, and/or combinations of all the above, could contribute to the observed power output.
Mitochondrial parameters such as transmembrane potential, respiration, calcium capacity, alterations in permeability transition susceptibility and ATPase activity were monitored.
Biochemical properties of particular importance included rate of respiration, glycolytic capacity and tolerance to hypoxic stress.
Animals were maintained under anesthesia with 1.5% isoflurane and 30% nitrous oxide and placed in a custom-designed stereotaxic animal holder outfitted with temperature and respiration monitoring capacity.
We demonstrated that in yeast limited in calorie supply the atg32Δ-dependent mutational block of macromitophagy reduces mitochondrial respiration, decreases capacity of the mitochondrial ETC, lowers the efficacy of coupling between ADP phosphorylation and electron transport, and disproportionally lessens activities of all OXPHOS enzymes.
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