The resulting bacterial suspension was mixed with equal amounts of two agrobacteria strains transformed with plasmids pICH14011 (encodes DNA integrase) and pICH10570 (5'-provector for cytosolic expression), respectively, prepared in the same way.
The cDNA sequences for each was amplified using primers described in Table 1, and cloned into two different bacterial expression vectors, pPROEX HTb (Life Technologies) and pGex‐KT (Hakes and Dixon, 1992), such that two recombinant fusion proteins (linked to hexa‐His and glutathione‐ S‐transferase (GST) tags, respectively) were prepared for each antigen.
Several subgroups were charged with, respectively, preparing the guidelines for methadone prescription, updating the methadone drug-drug interactions manual and designing an information booklet for the patient.
Four ordered mesoporous bioactive glasses (MBGs) with the same composition and hexagonal mesostructure but adjustable pore diameters (1.9, 3.4, 5.0, 6.4 nm, denoted MBGs A D, respectively) have been prepared for the first time by using different structure-directing agents as templates.
Hiei and Kurama are summoned by the other two rulers, Mukuro and Yomi, respectively, to prepare for an inevitable war.
The DL-DNA of the second or third generation with 12 or 24 highly potent CpG motifs in one unit, respectively, were designed and successfully prepared for the first time.
For the 3D cell cultures studied, furthermore, 2 kinds of culture models with different cell density levels (7.6 × 10 and 8.5 × 10 cells mL−1, indicated as " H" and " L," respectively, in Figure 5) were prepared for the chemosensitivity tests.
Two libraries, with 320 bp and 8 kbp insert size, were prepared for each strain, respectively.
Researchers at Oxford Instruments ('Lab 1') purchased 9 beef and 4 horse samples, from which 46 and 20 extracts were prepared for NMR analysis, respectively.
Two mixtures of equal amounts of the three banana root RNA samples for transcriptome analysis was prepared for each cultivar respectively using Illumina's kit, following the manufacturer's recommendations.
