The number of viable, apoptotic, and necrotic cells, which were stained with green fluorescence with intact nuclei, green fluorescence with the appearance of cell shrinkage, nuclear condensation and fragmentation, and bright orange fluorescence, respectively, were enumerated.
After incubation for ∼30 min, the "infected" Vero E6 monolayers were overlaid with MEM medium supplemented with methylcellulose (1%) and FCS (2%) for three days before fixing and staining by paraformaldehyde (4%) and crystal violet (2%), respectively, to enumerate the plaques for determining the percentage of infected cells at individual time points.
The two characteristics of the crowding effect were demonstrated graphically and, by interpolation, the estimated crowding thresholds were 903, ≤16, 39, ≤14, ≤16, ≤16 or 72 sporulated oocysts, respectively, for the seven Eimeria species enumerated above.
Both absorption spectrophotometric and steady-state fluorescence studies reveal effective and selective interaction between fullerenes and 1 as average binding constants (K) for the C60/1 and C70/1 complexes are enumerated to be 34,700 and 359,925 dm3 mol−1, respectively.
Asexual parasites and gametocytes were enumerated against 200 and 500 leukocytes, respectively, and expressed as density/µL assuming an average leukocyte count of 8,000/µL.
The grown colonies were examined and enumerated on the 21st and 30th days, respectively [ 43].
Egg masses (n = 20 and 37, contaminated site and reference site, respectively) were returned to the laboratory, where they were enumerated and a subsample of 100 fertile eggs were set aside for assessments of development.
If we have q datasets consisting of n1, n2, …, n q records, respectively, then the total number of q-tuples to be enumerated is n 1 × n 2 × …, × n q.
Haemolytic E. coli and coliform bacteria were enumerated on blood agar (Oxoid) and MacConkey agar (Merck), respectively, after aerobic incubation at 37°C for 1 d, whereas lactic acid bacteria (LAB) were enumerated on de Man Rogosa Sharpe agar (Merck) after anaerobic incubation at 37°C for 3 d.
Then, the LAB3 cells population decreased: 107 and 106 CFU mL−1 were enumerated after 12 days in alginate-caseinate matrix and in alginate matrix, respectively.
