Potentiometric titrations were confirmed by UV/Vis titrations of the respective combinations of amino acids and Co-imidazole.
Redox titrations were performed with 1 μg His-tagged Rap2.4a according to [ 6] in 150 μl MES buffer (100 mM) supplemented with 10 mM mixtures of oxidized and reduced DTT adjusted to the respective redox potential by redox titration.
Briefly, titrations of tetrapeptide Ac-FHTA-NH2 (KD=250 μ m), the wild-type RadA oligomerisation peptide (KD=7.0 μ m), and ATP (KD=2.5 μ m) were performed individually against apo protein to measure their respective KD values (top row titrations, Figure 2 B).
The basicity analyses of the respective catalysts were determined by benzoic acid titration using the following indicators: bromothymol blue (H− = 7.2), phenolphthalein (H− = 8.2 9.8), 2,4-dinitroaniline (H− = 15.0) and 4-nitroaniline (H− = 18.4) [25, 25].
The respective advantages and disadvantages of end-point titration and lag time assays are well known from assays of prion infection in vivo.
> -wrap-foot> Nofe of the beta-blockers was superior with regards to tolerability according to the primary endpoint of reaching the respective target doses when following the recommended titration scheme (Table 2 ).
When comparing the speciations of Fig. 9a, b, it can be seen that the share of CoL is ca 40%% and the share of CoL2 up to 90%% in the absence of imidazole, whereas in the titrations with [Co imid)2]n the respective values are 20 and 60%%.
The rate of reactions between adsorbed hydrogen and adsorbed ketones and the respective alcohols intermediates of hydrogenation have been obtained from the titration curves at different organic reagent concentrations.
At each point in the titration, the intermolecular PREs were summed over their respective residues and normalized to the highest value of each titration curve.
The respective insulin dose was estimated according to standard treatment recommendations and up-titrated according to a forced titration schedule (14), seeking a target fasting glucose level of <100 mg/dL.
