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In both protocols, no correlation between baseline serum or plasma BDNF and FFA and between serum or plasma BDNF and FFA at the respective time points during all experiments was observed.
While it is difficult to determine the frequency of abnormal migration phenotypes in the live-imaging analyses, we assume that cells with positional defects observed in Fig 4C, 5A, 6B and 7A represent snapshots at the respective time points during normal or abnormal migration.
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The choice of using different dyes at various time points during the experiment in cells, when protein translation is not inhibited, will lead to distinct populations of otherwise identical proteins whose discriminating features are determined through the time point of the respective labeling of each population.
In addition, the age-based data had more missing value than the visits-based due to the constraint of age to be identical during the respective time points.
Data from each chip were collated with respective time points into a LD and DD file respectively.
Data are presented as mean±s.e., *P<0.05, **P<0.01 and ***P<0.001 for differences between diet groups at respective time points based on estimated marginal means.
Native brains and spinals cord were prepared at the respective time points and snap-frozen at −70°C.
From the respective time points onward hyphal morphology became heterogeneous.
0.1 Hz TS-stimulation-evoked EPSC amplitudes were analyzed during a 5-minute control interval and in segments of 5 10 minutes LM22A-4, 10–15 minutes LM22A-4 and 15 20 minutes LM22A-4, and respective time points in the presence of K252a.
Briefly, treated cells were trypsinized and resuspended in the medium at respective time points.
The classification was based on signals for the respective time points.
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