Data from each chip were collated with respective time points into a LD and DD file respectively.
In contrast, pre-treatment with SHT significantly reduced p-PKA by 6, 89, and 69% at 15, 30, and 60 min after α-MSH stimulation, respectively, and p-CREB by 90, 91, and 65% at the respective time points compared with levels in cells treated with α-MSH alone.
This was significantly higher than for the larvae of control origin that showed a survival of 33% and 5% at these respective time points.
Average blood glucose levels, age at surgery, weight at surgery, weight at death, and weight change for all four treatment groups showed no statistical differences when compared within their respective time points (Table 2).
Though the optical density values of formazan solutions from the SF membrane group were lower than those from the control group at respective time points (Fig. 4), these differences were not statistically significant (P > 0.05).
The viability of cells cultured on SF membranes (SF group) and on control surfaces (control group) increased over time (P < 0.05); however, at respective time points, cell viability was not significantly different between the two groups (P > 0.05).
Native brains and spinals cord were prepared at the respective time points and snap-frozen at −70°C.
The median of ratios of individual microarrays was adjusted to be the logarithm of the estimated DNA content at respective time points from equation (2).
To determine DNA copy number increase at various loci, individual microarrays were normalized based on the DNA content at respective time points as described elsewhere [6].
The mean OD values were calculated for each dose at the respective time points and the percent survival as a function of time and dose was used to compare the experimental and control groups.
Values shown in Figure S1 and S2 were the average of triplicate real-time PCR reactions, normalized to the test gene expression in the control at the respective time points.
