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The medium containing the respective supplements was replaced every 2 days.
Consequently, we also removed Figure 10C, the respective supplements 5 and 6 as well as their legends and the reference to the mutants in the Materials and Methods section.
Table 1 (murine cells) and Table 2 (human cells) summarize these results while their respective supplements (Supplementary files 1, 2) show the inducibility of Perforin-2′s mRNA and protein (qPCR of ΔCT of Perforin-2 normalized to GAPDH and western blot analysis).
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Patients in the supplement group received the respective supplement post-HD (providing 500 kcal and 15 g protein) for 1 month in addition to the monitored diet prescription.
Because of this incomplete cross-over, although clinical characteristics of the two groups were not significantly different at baseline (Table 1), some were significantly different at the start of the respective supplement periods (week 0 for group 2, week 20 for group 1).
The data of genes having IRESs was obtained from http://iresite.org/ [ 13] and from [ 17], and its respective supplement: http://www.sciencemag.org/content/317/5842/1224/suppl/DC1.sciencemag.org/content/317/5842/1224/suppl/DC1
Human salivary gland (HSG) cells and SHSY-5Y neuroblastoma cells were cultured in their respective medium along with various supplements, as previously described.
Thereafter cells were maintained as for SP analyses except, before fixation, they were treated with their respective media supplemented with 2 µM cytochalasin D (Sigma-Aldrich Co., St . Louis Mo) for 14 hours to induce binucleated cell formation.
The inoculum was aspirated and A549 or MDCK cells were incubated in respective media supplemented with 0.2% BSA and antibiotics.
The respective media supplemented with 0.1% BSA, 5 mg l 1 transferrin, 5 μg l 1 selenium, penicillin G (100 units ml 1), and streptomycin (100 μg ml 1) were used as serum-free media.
HepG2 and Hep3B cells were cultured in respective media supplemented with 100 U/mL penicillin, 100 U/mL streptomycin, and 10% foetal bovine serum (FBS) and kept at 37°C in a humidified atmosphere of 5% CO2.
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